This Week in Virology

Hosts: Vincent Racaniello and Kathy Spindler Guests: Michael Imperiale, Adam Lauring, and Akira Ohno Vincent visits the University of Michigan where he and Kathy speak with Michael, Adam, and Akira about polyomaviruses, virus evolution, and virus assembly, on the occasion of naming the department of Microbiology & Immunology a Milestones in Microbiology site. Links for this episode World Polio Day 3:20 Mole Day 5:25 The rat virus (J Inf Dis) 20:00 Polyomavirus miRNAs (Curr Op Virol) 39:30 Costs of GOF pause (mBio) 45:00 Lethal mutagenesis of influenza virus (J Virol) 1:03:35 Mutational robustness in RNA virus evolution (Nat Rev Micro) 52:00 Membrane binding of gag (mBio) 1:12:55 Cell proteins in retrovirus particles (J Virol) 1:23:15 Michael's twitter Adam's twitter Video of this episode - view below or at YouTube Weekly Science Picks 1:27:30 Kathy - Ada Lovelace and Charles Babbage Lego #1 and #2 and Ada Lovelace DayVincent - MiSciWriters Listener Picks Junio - XKCD marks the spotKari - Spoonflower Send your virology questions and comments to twiv@twiv.tv

Host: Vincent Racaniello Guest: Blossom Damania Vincent speaks with Blossom about her laboratory's research on Kaposi's sarcoma-associated herpesvirus, including how it transforms cells, the switch between lytic and latent replication, and its interaction with the innate immune system of the host. Links for this episode Damania Laboratory Modulation of cGAS-STING by KSHV (PNAS) KSHV vIRF1 interacts with ISG15 pathway member (J Virol) KSHV NLR homolog inhibits inflammasome (Science) KSHV inhibition of PI3K and mTOR (Blood) KSHV confers survival advantage to endothelial cells (Cancer Res) UNC Global Oncology Program Blossom on Twitter Video of this episode - view at YouTube Send your virology questions and comments to twiv@twiv.tv

Host: Vincent Racaniello Guest: Ileana Cristea Vincent meets up with Ileana at Princeton University to talk about how her laboratory integrates molecular virology, mass spectrometry-based proteomics, and bioinformatics to unravel the interplay between virus and host. Links for this episode Cristea Laboratory Ileana's work previously on TWiV 269 Blowing off steam (Cell Host Micr) Nuclear viral DNA sensors (J Biol Chem) mSystems Video of this episode - view at YouTube (coming soon!) Send your virology questions and comments to twiv@twiv.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guest: Jens Kuhn Jens joins the TWiVomics to discuss the strengths and weaknesses of viral taxonomy, including its history and evolution, how viruses are ordered, and why T. rex was classified without having a living isolate. Links for this episode Jens' previous TWiV (283) 5:15 International Committee on the Taxonomy of Viruses (ICTV) 27:50 ICTV 9th Report 32:55 Classifying viruses by capsid architecture (Curr Opin Virol) 1:43:00 Image credit Letters read on TWiV 357 10:55, 1:55:40 Timestamps by Jennifer. Thanks! Weekly Science Picks 56:30, 2:07:15 Alan - The Jefferson GridRich - Summer of ScienceKathy - Bill Nye dances The ClickDickson - The MartianJens - How I Killed Pluto, Life in Our Phage World, Why the Seahorse Tail is SquareVincent - Does the public trust science? (videos, summaries) Send your virology questions and comments to twiv@twiv.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guest: Stephanie Neal Stephanie joins the super professors to discuss the gut virome of children with serious malnutrition, caterpillar genes acquired from parasitic wasps, and the effect of adding chemokines to a simian immunodeficiency virus DNA vaccine. Links for this episode Invitrogen Science Hero Awards 15:05 Gut DNA viromes of twins with severe malnutrition (PNAS) 34:30 Random forests 48:00 Wasps engineer caterpillars (PLoS Genetics) 50:20 Chemokine-adjuvanted SIV DNA vaccine (Mucos Immunol) 1:02:20 Guide to chemokines (pdf) Role for CCR10 in IgA secreting cell accumulation (J Immunol) CCL28 controls IgA plasma cell accumulation (J Exp Med) In vivo electroporation 1:07:25, 1:09:50 Kathy's groan (jpg) Image credit Letters read on TWiV 356 14:55 Weekly Science Picks 1:18:35, 1:45:30 Alan - Plates from Hooke's MicrographiaRich - The Wright Brothers by David McCulloughKathy - Spore evaporation-driven engines (video and article)Dickson - Color variations of Pluto and Lunar total eclipseStephanie - The science of moldy cheese by Carl ZimmerVincent - But Why? Listener Pick of the Week Reed - The Bench Warmers Podcast Send your virology questions and comments to twiv@twiv.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The TWiV team considers the effect of a Leishmaniavirus on the efficacy of drug treatment, and the human fecal virome and microbiome in twins during early infancy. Links for this episode Announcing mSphere 5:50 Leishmania virus influences drug outcome (paper 1, paper 2) 25:15 Human gut virome and microbiome in infants (Nat Med) 46:00 Multiple displacement amplification (Wiki) 46:00 Virome of adult twins (Nature) 49:15 Illumination by Kevin Folta 1:31:00 Folta story at RetractionWatch 1:41:40 Image credit Letters read on TWiV 355 9:40, 1:20:30 This episode is sponsored by ASMGAP 5:00 Timestamps by Jennifer. Thanks! Weekly Science Picks 1:42:20 Alan - Vexed Muddler storeRich - Ivanpah Solar Power FacilityKathy - The real KermitDickson - Animal PortraitsVincent - Adventures of the Regatjes, Vaccines by Susan Nasif Listener Pick of the Week Jacob - Great Wall of China keeps rabbits out Send your virology questions and comments to twiv@twiv.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The esteemed doctors of TWiV review a new giant virus recovered from the Siberian permafrost, why influenza virus gain of function experiments are valuable, and feline immunodeficiency virus. Links for this episode Non-viral proteins in vaccinia virus particles (Arch Virol) 8:15 Thing a week (Jonathan Coulton, downloads) 23:20 Immortal Life pornographic? Not! (HuffPost) 25:25 Mollivirus sibericum (PNAS) 27:00 Don't worry about Frankenvirus (WashPost) 27:10 We need flu GOF research (article, Wisc State J, Science) 40:35 Isolation of FIV (Science) 50:20 FIV at Cornell Vet 1:06:25 FIV latency (Retrovirol) 1:08:00 FIV-receptor interactions (Curr Op Virol) 1:08:45 Clin aspects FIV infection (Viruses) FIV antivirals (Viruses) 1:10:05 How to treat FIV 1:10:45 Bud's FIV therapy 1;10;50 FIV vectors (CSH Prot) 1:13:50 Lion FIV (Proc Biol Sci) FIV in cat species (J Virol) 1;01;45 ACIP HVP vaccine recommendations (MMWR) 1:28:45 HPV and cancer (CDC) 1:33:45 Cervical cancer screening (pdf, CDC) Letters read on TWiV 354 7:45, 1:17:10 This episode is sponsored by ASMGAP Timestamps by Jennifer. Thanks! Weekly Science Picks 1:40:40 Alan - Experience CuriosityRich - Primrose SchoolKathy - Egg drop solutionsDickson - Dragonflies by Pieter van DokkumVincent - NASA guide to air-filtering houseplants Listener Pick of the Week Amir - Publons (Nature article) Send your virology questions and comments to twiv@twiv.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler The TWiVniacs discuss twenty-eight years of poliovirus shedding by an immunodeficient patient, and packaging of the innate cytoplasmic signaling molecule cyclic GMP-AMP in virus particles. Links for this episode 28 years of poliovirus shedding (PLoS Path) 36:50 NOT a rare poliovirus (Wash Post) 44:35 Ukraine polio outbreak (ProMedMail) 41:00 Virus particle transfer of cGAMP (Science one, two) 47:50 cGAS on TWiV 222 47:05 Image credit Letters read on TWiV 353 6:30, 1:10:25 This episode is sponsored by ASMGAP 3:10 Timestamps by Jennifer. Thanks! Weekly Science Picks 1:27:50 Alan - Miniature calendarKathy - Dr. Brainlove (prologue, v. 2.0, IndieGoGo)Dickson - The Ph.D. MovieVincent - Ghostery Listener Pick of the Week Ramon - Children dieChris - ASMLive at ICAAC/ICC Send your virology questions and comments to twiv@twiv.tv

Host: Vincent Racaniello Guest: Michele Banks Vincent meets up with Michele Banks in Washington, DC to discuss her career as a creator of science-themed art. Links for this episode Michele Banks on Twitter Artologica Michele's blog The Finch and the Pea Joseph Cornell Not Exactly Rocket Science (Ed Yong) Tree of Life (Jonathan Eisen) Home Microbiome Study Kitten Microbiome Project Science Online The Vexed Muddler Luke Jerram A Daily Dish (Klari Reis) Neuroscience art (Greg Dunn) Ai Weiwei Video of this episode - coming soon Video of Michele working in her studio - view at YouTube Send your virology questions and comments to twiv@twiv.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The Masters of the ScienTWIVic Universe discuss a novel poxvirus isolate from an immunosuppressed patient, H1N1 and the gain-of-function debate, and attenuation of dengue virus by recoding the genome. Links for this episode Paula Pitha-Rowe (Balt Sun) Novel poxvirus (CID) H1N1 and gain-of-function debate (mBio) Recoding dengue virus genome (PNAS) Attenuation by dinucleotide frequency change (PNAS) Codon pair and dinucleotide bias not distinguished (PNAS) Codon pair deoptimization artefact of CG/UA change (eLife) Can engineering subdue viral vaccine reversion? (Virus Evol) Attenuation by a thousand cuts (NEJM) Image credit Letters read on TWiV 351 Weekly Science Picks Alan - Genetic roulette Rich - The Huntington Kathy - Simulated vomiting machine (video) Dickson - Last Cassini Saturn flybyVincent - Decaying Still Life (YouTube) Listener Pick of the Week Ken - Cave meterology (Times, Nature) Send your virology questions and comments to twiv@twiv.tv