This Week in Virology

TWiV explains genetic changes in nOPV2 strains that paralyzed children, outbreak of raccoon distemper in Toronto, EcoHealth Alliance gets its NIH grant back, and breakage at chromosomal fragile sites caused by the EBNA1 protein of Epstein-Barr virus as a mechanism for carcinogenesis. Hosts: Vincent Racaniello, Rich Condit, Kathy Spindler, and Angela Mingarelli Subscribe (free): Apple Podcasts, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Register for ASV 2023 Research assistant position at FDA (pdf) MicrobeTV Discord Server nOPV2 after two years (Polioeradication) Raccoon distemper in Toronto (CityNews Everywhere) NIH reinstates EcoHealth grant (Nature) Chromosomal breakage by EBNA1 (Nature) Agent of chromosomal instability (Nature) Timestamps by Jolene. Thanks! Weekly Picks Angela – Night Science Kathy – Kermit, Jim Henson, Rainbow Connection Rich – Alexander Skarsgård Explains the Answer to Everything Vincent – Beyond the Noise with Dr. Paul Offit Intro music is by Ronald Jenkees Send your virology questions and comments to twiv@microbe.tv

In his weekly clinical update, Dr. Griffin discusses immunogenicity and tolerability of a bivalent virus-like particle norovirus vaccine candidate in children from 6 months up to 4 years of age, influenza hemagglutinin stem nanoparticle vaccine induces cross-group 1 neutralizing antibodies in healthy adults, COVID-19 surveillance after expiration of the public health emergency declaration, provisional mortality data, targeted vaccine messaging to promote COVID-19 vaccines for children and youth, vaccines and related biological products advisory committee meeting June 15, 2023 announcement, how to overhaul the CDC, impact of SARS-CoV-2 variants on inpatient clinical outcome, prevalence of post-coronavirus disease condition 12 weeks after Omicron infection compared with negative controls and association with vaccination status, gut bacteria cocktail helps long COVID, and gut microbiota‐derived symbiotic formula (SIM01) as a novel adjuvant therapy for COVID‐19. Subscribe (free): Apple Podcasts, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Immunogenicity and tolerability of a bivalent norovirus vaccine in children (HVI) Influenza hemagglutinin stem nanoparticle vaccine induces neutralizing antibodies in healthy adults (STM) COVID-19 surveillance after expiration of the public health emergency declaration (CDC) Provisional mortality data (CDC) Targeted messaging to promote COVID-19 vaccines for children and youth (Pediatrics) Vaccines and biological products advisory committee meeting announcement (FDA) How to overhaul the C.D.C (NYTIMES) Impact of SARS-CoV-2 variants on inpatient clinical outcome (CID) Prevalence of post-COVID19 condition 12 weeks after Omicron infection and vaccination status (CID) Gut bacteria cocktail helps long COVID (MedPage) Gut microbiota‐derived synbiotic formula as a therapy for COVID‐19 (NIH) Contribute to our FIMRC fundraiser at PWB Letters read on TWiV 1006 Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees Send your questions for Dr. Griffin to daniel@microbe.tv

TWiV reveals plankton-infecting relatives of herpesviruses in the sunlit oceans, and a vaccine encoding non-spike T cell antigens that protects animals from severe SARS-CoV-2 infection. Hosts: Vincent Racaniello, Alan Dove, Kathy Spindler, and Brianne Barker Subscribe (free): Apple Podcasts, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Register for ASV 2023 Research assistant position at FDA (pdf) MicrobeTV Discord Server Mirusviruses link herpesvirus to giant viruses (Nature) Tara Oceans expeditions T cell vaccine protects against severe COVID (Cell) Letters read on TWiV 1005 Timestamps by Jolene. Thanks! Weekly Picks Brianne – Immune #67: Can T cells Live Forever? Kathy – Hazel Scott, Trinidad Jazz star Alan – The Last Navigator, Steve Thomas Vincent – Nasal Spray Covid Vaccines: Hope or Hype? Intro music is by Ronald Jenkees Send your virology questions and comments to twiv@microbe.tv

In his weekly clinical update, Dr. Griffin discusses the U.S. Food and Drug Administration approving Arexvy: the first respiratory syncytial virus (RSV) vaccine approved for use in the United State, association between SARS-CoV-2 and metagenomic content of samples from the Huanan Seafood Market, researchers disagree over how bad it is to be reinfected and whether COVID-19 can cause lasting changes to the immune system, virtual care and emergency department use during the COVID-19 pandemic among patients of family physicians in Canada, vaccination with BCG-Denmark did not result in a lower risk of COVID-19 among health care workers than placebo, NVX-CoV2373 vaccine efficacy against hospitalization, comparative effectiveness of the SARS-COV-2 vaccines during Delta dominance, assessment of gender-specific COVID-19 case fatality risk per malignant neoplasm type, clinical outcomes following treatment for COVID-19 with Nirmatrelvir/Ritonavir and Molnupiravir among patients living in nursing homes, timing of intubation and ICU mortality in COVID-19 patients, real-life experience with Remdesivir for treatment of COVID-19 among older adults, and how long COVID brain fog and muscle pain are associated with longer time to clearance of SARS-CoV-2 RNA from the upper respiratory tract during acute infection. Subscribe (free): Apple Podcasts, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode FDA approves Arexvy, the first RSV vaccine approved in the US (FDA) Association between SARS-CoV-2 and content of samples from the Huanan Seafood Market (bioRxiv) Researchers disagree over how bad it is to be reinfected with COVID-19 and lasting changes to the immune system (Nature) Virtual care and emergency department use during the COVID-19 Pandemic among patients of family physicians (JAMA) Vaccination with BCG-Denmark did not result in a lower risk of COVID-19 among health care workers (NEJM) NVX-CoV2373 vaccine efficacy against hospitalization (Vaccine) Comparative effectiveness of the SARS-COV-2 vaccines during Delta (Cell) Assessment of gender-specific COVID-19 case fatality risk (JAMA) Clinical outcomes following treatment for COVID-19 with Nirmatrelvir/Ritonavir and Molnupiravir (JAMA) Timing of intubation and ICU mortality in COVID-19 patients (BMC) Real-life experience with Remdesivir for treatment of COVID-19 (JAC) Long COVID brain fog and muscle pain are associated with longer time to clearance of SARS-CoV-2 RNA (Frontiers) Contribute to our FIMRC fundraiser at PWB Letters read on TWiV 1004 Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees Send your questions for Dr. Griffin to daniel@microbe.tv

Vincent and Kathy travel to Atlanta for APHL ID Lab Con, a conference focused on the latest developments in detecti​​on and characterization of infectious diseases of public health concern, and speak with Megan, Ryan, and Kirsten about the workings of public health and clinical laboratories. Hosts: Vincent Racaniello and Kathy Spindler Guests: Megan Crumpler, Ryan Relich, and Kirsten St. George Subscribe (free): Apple Podcasts, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode MicrobeTV Discord Server APHL Lab ID CON Association of Public Health Laboratories Orange County Public Health Laboratory Division of Clinical Microbiology, Indiana University Health Virology Laboratory, NYSDOH Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees Send your virology questions and comments to twiv@microbe.tv

In his weekly clinical update, Dr. Griffin discusses completeness and spin of medRxiv preprints and associated published abstracts of COVID-19 randomized clinical trials, lessons learned from a COVID-19 dog screening pilot in California K-12 schools, SARS-CoV-2 reinfection and severity of the disease, effectiveness of COVID-19 bivalent vaccine, Molnupiravir and risk of post-acute sequelae of COVID-19, risk of new-onset long Covid following reinfection with SARS-CoV-2, low vitamin D levels associated with long COVID syndrome in COVID-19 survivors, and clinical experience with the α2A-adrenoceptor agonist, guanfacine, and N-acetylcysteine for the treatment of cognitive deficits in "Long-COVID19". Click arrow to play Download TWiV 1002 (27 MB .mp3, 44 min) Subscribe (free): Apple Podcasts, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Completeness and spin of medRxiv preprint and published abstracts of COVID-19 randomized clinical trials (JAMA) Lessons learned from a COVID-19 dog screening pilot (JAMA) SARS-CoV-2 reinfection and severity of the disease (Viruses) Effectiveness of COVID-19 bivalent vaccine (medRxiv) Molnupiravir and risk of post-acute sequelae of COVID-19 (BMJ) Risk of new-onset long Covid following reinfection with SARS-CoV-2 (medRxiv) Low vitamin D levels are associated with Long COVID in COVID-19 survivors (JCEM) Clinical experience with the α2A-adrenoceptor agonist, guanfacine, and N-acetylcysteine for treatment of Long-COVID19 (NIH) Contribute to our ASTMH fundraiser at PWB Letters read on TWiV 1002 Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees Send your questions for Dr. Griffin to daniel@microbe.tv

In his weekly clinical update, Dr. Griffin discusses an update on vaccine-derived poliovirus outbreaks, how the dream of wiping out polio might need a rethink, risk of death in patients hospitalized for COVID-19 vs seasonal Influenza in fall-winter, French Mpox cluster includes fully vaccinated patients, two individuals with potential Mpox virus reinfection, epidemiologic and clinical features of Mpox-associated deaths, FDA authorizes changes to simplify use of bivalent mRNA COVID-19 vaccines, SARS-CoV-2 during Omicron variant predominance among infants born to people with SARS-CoV-2, severe maternal morbidity and mortality of pregnant patients with COVID-19 infection during the early pandemic period in the US, sickness presenteeism in healthcare workers during the COVID-19 pandemic, ventilation improvements among k–12 public school districts, risk factors and vectors for SARS-CoV-2 household transmission, SARS-CoV-2 neutralizing antibodies after bivalent versus monovalent, durability of bivalent boosters against Omicron subvariants, Nirmatrelvir and risk of hospital admission or death in adults with COVID-19, evolving real-world effectiveness of monoclonal antibodies for treatment of COVID-19, effect of angiotensin-converting enzyme inhibitor and angiotensin receptor blocker initiation on organ support–free days in patients hospitalized with COVID-19, efficacy and safety of Anakinra plus standard of care for patients with severe COVID-19, higher dose corticosteroids in patients admitted to hospital with COVID-19 who are hypoxic but not requiring ventilatory support, risk of autoimmune diseases in patients with COVID-19, definition of post–COVID-19 condition among published research studies, and sleep disturbance severity and correlates in post-acute sequelae of COVID-19. Subscribe (free): Apple Podcasts, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Update on vaccine-derived poliovirus outbreaks (CDC) The dream of wiping out polio might need a rethink (NPR) Risk of death in patients hospitalized for COVID-19 vs influenza (JAME) French mpox cluster includes fully vaccinated patients (CIDRAP) Two individuals with potential Monkeypox reinfection (The Lancet) Epidemiologic and clinical features of Mpox-associated deaths (CDC) FDA authorizes changes to simplify use of Bivalent mRNA COVID-19 vaccines (FDA) Omicron variant predominance among infants born to people with SARS-CoV-2 (Pediatrics) Severe maternal morbidity and mortality of pregnant patients with COVID-19 (JAMA) Sickness presenteeism in healthcare workers during the COVID-19 pandemic (ICHE) Ventilation improvements among K–12 public school districts (CDC) Risk factors and vectors for SARS-CoV-2 household transmission (The Lancet) SARS-CoV-2 neutralising antibodies after bivalent vs monovalent (The Lancet) Durability of bivalent boosters against Omicron subvariants (NEJM) Nirmatrelvir and risk of hospital admission or death in adults with COVID-19 (BMJ) Real world effectiveness of Monoclonal Antibodies for treatment of COVID-19 (AIM) Study on ACE inhibitor and ARB effect on COVID-19 patient outcomes (JAMA) Efficacy and safety of Anakinra plus standard of care for patients with severe COVID-19 (JAMA) High-dose corticosteroids in non-ventilated COVID-19 patients with hypoxia (The Lancet) Risk of autoimmune diseases in patients with COVID-19 (eClinical) Definition of post–COVID-19 condition (JAMA) Sleep disturbance severity and correlates in Post-acute Sequelae of COVID-19 (JGIM) Contribute to our ASTMH fundraiser at PWB Letters read on TWiV 1001 Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees Send your questions for Dr. Griffin to daniel@microbe.tv

The entire TWiV team gathers at a theatre in New York City to celebrate a milestone in science podcasting, to reminisce how they joined the show, and to play a game of TWiV Jeopardy. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, Kathy Spindler, Brianne Barker, Daniel Griffin, and Angela Mingarelli Subscribe (free): Apple Podcasts, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode TWiV #1: West Nile virus TWiV #100: TWiV catches a big fish TWiV #200: Threading the NEIDL TWiV #300: So happy together TWiV #400: Harold '400' Varmus, a scientist for all seasons TWiV #500: Keep virology weird Randal Despommier Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees Send your virology questions and comments to twiv@microbe.tv

TWiV notes Ashish Jha's call for doctors to combat a vacuum of medical information while ignoring our contributions, China CDC's surveillance of SARS-CoV-2 at the Huanan Seafood Market, and an experimental infectious attenuated COVID-19 vaccine that elicits superior mucosal and systemic immunity in hamsters. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Kathy Spindler, and Brianne Barker Subscribe (free): Apple Podcasts, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Register for ASV 2023 MicrobeTV Discord Server Jha ignores TWiV contribution to disseminating science information (STAT) Surveillance of SARS-CoV-2 at the Huanan Market (Nature) Pandas in the Huanan Market? (ArsTechnica) Infectious attenuated COVID-19 vaccine candidate (Nature Micro) Virus attenuation by codon pair deoptimization (Cell Rep) Timestamps by Jolene. Thanks! Weekly Picks Dickson – Jazz Project: Conductor/composer: Gill Evans. Produced Sketches of Spain, Porgy And Bess. Quincey Jones. Numerous albums, all kinds of music. Duke Ellington. Prolific musician, pianist, and composer. Antonio Carlos Jobim. Signature album: Sound track from the film – Black Orpheus. Signature songs: Girl From Ipanema; The Waters of March. Brianne – This is what it sounds like when plants cry Kathy – I know who you are by Barbara Rae-Venter Alan – Svalbard seed vault virtual tour Vincent – We're Asking the Impossible of Vaccines by Katherine Wu Intro music is by Ronald Jenkees Send your virology questions and comments to twiv@microbe.tv

In his weekly clinical update, Dr. Griffin discusses the White House COVID adviser calling on doctors to combat a vacuum of medical information, adeno-associated virus type 2 in US children with acute severe hepatitis, genomic investigations of unexplained acute hepatitis in children, surveillance of SARS-CoV-2 at the Huanan seafood market, vaccine effectiveness against hospitalization among adolescent and pediatric SARS-CoV-2 cases between May 2021 and January 2022 in Canada, effectiveness of BNT162b2 after extending the primary series dosing interval in children and adolescents aged 5–17, prevalence and characteristics associated with post–COVID-19 condition among non-hospitalized adolescents and young adults, within-host genetic diversity of SARS-CoV-2 lineages in unvaccinated and vaccinated individuals, live-attenuated vaccine sCPD9 elicits superior mucosal and systemic immunity to SARS-CoV-2 variants in hamsters, Fc-γR-dependent antibody effector functions are required for vaccine-mediated protection against antigen-shifted variants of SARS-CoV-2, COVID-19 convalescent plasma utilization in the United States, FDA authorizes Gohibic (vilobelimab) injection for the treatment of COVID-19, anti-C5a antibody IFX-1 (vilobelimab) treatment versus best supportive care for patients with severe COVID-19 (PANAMO), anti-C5a antibody (vilobelimab) therapy for critically ill, invasively mechanically ventilated patients with COVID-19, triple combination therapy with two antivirals and monoclonal antibodies for persistent or relapsed SARS-CoV-2 infection in immunocompromised patients, and the breadth of the neutralizing antibody response to original SARS-CoV-2 infection is linked to the presence of long COVID symptoms. Subscribe (free): Apple Podcasts, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode White House COVID adviser calls on doctors to combat medical information (STAT) Adeno-associated virus type 2 in US children with acute severe hepatitis (Nature) Genomic investigations of unexplained acute hepatitis in children (Nature) Surveillance of SARS-CoV-2 at the Huanan Seafood Market (ChinaXiv) Vaccine effectiveness against hospitalization among pediatric SARS-CoV-2 cases (PLOS) Effectiveness of BNT162b2 after extending the series dosing interval in children (Nature) Prevalence and characteristics associated with post–COVID-19 condition (JAMA) Within-host genetic diversity of SARS-CoV-2 lineages in unvaccinated and vaccinated individuals (Nature) Replication-competent-attenuated vaccine sCPD9 elicits immunity to SARS-CoV-2 variants (Nature) Fc-γR antibody functions needed for SARS-CoV-2 variant protection via vaccination (Nature) COVID-19 convalescent plasma utilization in the United States (CID) FDA authorizes Gohibic injection for the treatment of COVID-19 (FDA) IFX-1 antibody vs. supportive care for severe COVID-19 patients (Lancet) Vilobelimab therapy for COVID-19 critically ill patients on mechanical ventilation (Lancet) Triple combo therapy for relapsed SARS-CoV-2 in immunocompromised patients with antivirals and antibodies (CID) Neutralizing antibody response breadth to SARS-CoV-2 tied to Long COVID presence (medRxiv) Neurological Long COVID pathogenesis and potential therapies (Journal Cells) Contribute to our ASTMH fundraiser at PWB Dr. Griffin's treatment guide (pdf) Letters read on TWiV 998 Don't crush Paxlovid (pdf) Timestamps by Jolene. Thanks! Intro music is by Ronald Jenkees Send your questions for Dr. Griffin to daniel@microbe.tv