The Stem Cell Podcast

Guest: Stem cell researcher and politician Dr. Raj Kittappa to discuss his political expertise as well as his latest research endeavors with stem cells and Parkinson’s disease. Resources and Links Wine Being Made 6300 Years Ago – Researchers have uncorked the oldest solid evidence of grape-based wine making in Europe, and possibly the world, at a site in northern Greece using a new method of analyzing the chemicals in liquids absorbed by clay containers. Scientists Have Gained Insight to Why Mitochondrial DNA Is Passed Down to Children by Their Mothers — But Not Their Fathers – DNA inside energy-producing organelles called mitochondria is destroyed in a dad’s sperm shortly after it fertilizes an egg. A New Cause of Lyme Disease – This article reveals that a new species of bacteria is causing Lyme disease, adding to worries that the infection will continue its relentless escalation across the United States. Functional Coupling with Cardiac Muscle Promotes Maturation of hPSC-Derived Sympathetic Neurons – Researchers derive sympathetic neurons from human pluripotent stem cells (hPSCs) and show that they can form physical and functional connections with cardiac muscle cells. In Vivo Tracking of Human Hematopoiesis Reveals Patterns of Clonal Dynamics during Early and Steady-State Reconstitution Phases – Scientists discovered that in vitro-manipulated hematopoietic stem/progenitor cells retain the ability to return to latency after transplant and can be physiologically reactivated, sustaining a stable hematopoietic output. Derivation of Diverse Hormone-Releasing Pituitary Cells from Human Pluripotent Stem Cells – This article presents a simple and efficient strategy to derive human pituitary lineages from human pluripotent stem cells using monolayer culture conditions suitable for cell manufacturing. Retinoic Acid Is Sufficient for the In Vitro Induction of Mouse Spermatocytes – Researchers report that retinoic acid is sufficient for inducing leptotene/zygotene spermatocytes from cultured mouse spermatogonial stem cells. Photo Reference: Courtesy of Dr. Raj Kittappa Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

Guest: Stem cell researcher Dr. Danwei Huangfu discusses her work and latest paper in Cell Stem Cell. Her team used CRISPR technology to genetically manipulate human pluripotent stem cells and induce pancreatic beta cells. Resources and Links FDA Warning about Imodium Ad Overdoses – The U.S. Food and Drug Administration (FDA) warned that higher-than-recommended doses of the antidiarrheal medicine loperamide, sold under the brand name Imodium, can lead to serious heart problems and even death. Who Advises Women to Delay Pregnancy over Zika Virus Threat – World Health Organisation (WHO) issues advice to millions of people in regions affected by virus that can cause severe birth defects and microcephaly. Sense of Touch and Autism – This article reveals that in mice, mutations in autism-related genes in skin nerve cells linked to anxiety, poor social skills. Four New Elements Now Have Names – Four elements that were officially added to the periodic table have been named as nihonium, moscovium, tennessine, and oganesson. Integrated Genomic Analysis of Diverse Induced Pluripotent Stem Cell from the Progenitor Cell Biology Consortium – The Progenitor Cell Biology Consortium performed comprehensive experimental and genomic analyses of 58 induced pluripotent stem cells from ten laboratories generated using a variety of reprogramming genes, vectors, and cells. A Cure for Multiple Sclerosis? – According to this article, chemotherapy, combined with stem cell transplants, could halt the onslaught of multiple sclerosis in people suffering from the disease. New Techniques to Assess the Fate of Stem Cells In Vivo – Researchers from the Université Libre de Bruxelles Cancer Research Center, led by Cédric Blanpain, developed new methods to assess with great precision the multipotent or unipotent fate of mammary gland and prostate stem cells. Generation of Mice with Longer and Better Preserved Telomeres in the Absence of Genetic Manipulations – This article reveals how researchers use mouse embryonic stem cells with ‘hyper-long’ telomeres, which also express green fluorescent protein, to generate chimaeric mice containing cells with both hyper-long and normal telomeres. Photo Reference: Courtesy of Dr. Danwei Huangfu Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

Guest: Stem cell researcher Dr. Kristin Hope discusses her work and latest paper in Nature. Her team examined umbilical cord blood-derived hematopoietic stem cells and the RNA-binding protein musashi-2. Resources and Links Doctors Say Olympics Should Be Postponed or Moved – Doctors and professors are urging the World Health Organization to move or postpone the summer Olympic games in Rio de Janeiro due to the widening Zika outbreak in Brazil. Bacteria Resistant to Last-Resort Antibiotic Appears in U.S. – This article reports that a 49-year-old woman in United States has tested positive for a strain of Escherichia coli, known as mrc-1, which is resistant to the antibiotic colistin and that the bacteria’s gene rides on a transferable loop of DNA called a a plasmid. Fruit Fly’s Giant Sperm Is Quite an Exaggeration – This article reveals that certain fruit fly species, such as Drosophila bifurca, males measuring just a few millimeters produce sperm with a tail as long as 5.8-centimeters. FDA Approves Device to Wean Addicts off Heroin – The U.S. Food and Drug Administration has approved a drug-emitting implant to combat addiction to heroin and other opioids killing thousands of people annually. Stem Cell Industry Blowing Up... The Global Market for Stem Cells – This article reports that the global stem cell market reached over $5.9 billion in 2015 and will grow from about $6.7 billion in 2016 to nearly $12.3 billion in 2021, registering a compound annual growth rate of 13.1% from 2016 through 2021. Israeli Firms Develop High-Speed 3D Printer for Stem Cells – Israeli 3D printer firm Nano Dimension has successfully lab-tested a 3D bioprinter for stem cells, paving the way for the potential printing of large tissues and organs. Biologists Find How Plants Reconstitute Stem Cells – A team of biologists at New York University showed that regenerating plants can naturally reconstitute their stem cells from more mature cells by replaying embryogenesis. Genetic Drift Can Compromise Mitochondrial Replacement by Nuclear Transfer in Human Oocytes – Researchers show that using human mitochondrial replacement stem cell lines, even though the low levels of heteroplasmy introduced into human oocytes by mitochondrial carryover during nuclear transfer often vanish, they can sometimes instead result in mtDNA genotypic drift and reversion to the original genotype. Photo Reference: Courtesy of Dr. Kristin Hope Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

Guest: Stem cell researcher and neuroscientist Dr. Su-Chun Zhang discusses his work and latest paper in Cell Stem Cell. His paper looks at grafting human pluripotent stem cells in a mouse model of Parkinson's disease. Resources and Links Scientists Talk Privately about Creating a Synthetic Human Genome – Scientists are now contemplating the fabrication of a human genome, meaning they would use chemicals to manufacture all the DNA contained in human chromosomes. Second Skin’ May Reduce Wrinkles, Eyebags, Scientists Say – Researchers said a “second skin” composed of commonly used chemicals like siloxanes can be painted on your skin and give it the elasticity of youth. Faulty Gene Can Turn Colds Deadly for Babies, Toddlers – This article reveals that some children have rare genetic variants that can turn their first encounter with colds and other respiratory viruses deadly. Australia Will Release Herpes into Its Rivers to Wipe Out Invasive Carp – This article reports that the Australian government plans to use a herpes virus to eradicate the country’s most devastating invasive species—common carp. The US Is about to Hit a Big Solar Energy Milestone – The US is on track to hit a big number in renewable energy: more than 1 million American homes should have solar panels by February 2016. Stem Cells Are Dangerously Overhyped, Warn Researchers – A study recently published says it’s time for everyone—from the media, to press officers, to journal publishers and even the scientists themselves—to chill out about stem cells. New Stem Cell Discovery Could Boost Corn Crop Yields by an Astounding 50 Percent – This article reports that a newly discovered stem cell signaling pathway could boost yields from corn and other staple crops by up to 50 percent in the very near term. Zika Virus Disrupts Neural Progenitor Development and Leads to Microcephaly in Mice – Researchers found that Asian Zika virus strain SZ01 could infect neural precursor cells in vivo and affect brain development; it replicates efficiently in embryonic mouse brain by directly targeting different neuronal linages. Zika Virus Depletes Neural Progenitors in Human Cerebral Organoids through Activation of the Innate Immune Receptor TLR3 – This article describes how Zika virus infection leads to microcephaly, using human embryonic stem cell-derived cerebral organoids to recapitulate early stage, first trimester fetal brain development. Why Lab-Made Stem Cells Might Fail: Errors in DNA Folding – Researchers have discovered one of the reasons why induced pluripotent stem cells don't always correctly differentiate back into adult cells: the reversion process does not always fully capture the way a cell's genome is folded up inside its nucleus. Photo Reference: Courtesy of Dr. Su-Chun Zhang Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

Guest: Stem cell researcher Dr. Arnold Kriegstein from the University of California, San Francisco to discusses his work and latest paper in Cell Stem Cell on how the Zika virus affects neural stem cells. Resources and Links Zika Is More Dangerous than We Thought – This article describes the danger of Zika virus like microcephaly in children born from women who have had Zika during pregnancy and Guillain-Barre syndrome – a nerve disorder that causes temporary muscle paralysis. Plants Use Prions to Remember – This article reveals that prions, proteins that change shape and shift tasks, may occur in plants as a form of memory. Uncertainty Is Stressful, but that Stress May Not Always Be Bad – This article demonstrates that subjective estimates of uncertainty predict the dynamics of subjective and physiological stress responses. Dragons Sleep Like Us – Sleeping lizards appear to share distinctive brain activity patterns with sleeping birds and mammals. EPA Boosts Estimate of U.S. Methane Emissions – This article reports that US. Environmental Protection Agency (EPA) has boosted its estimate of total U.S. methane emissions by 13 percent, which is an increase of more than 3.4 million metric tons of the greenhouse gas and has the same long-term global warming impact as a year’s worth of emissions from about 20 million cars. Risky Skull Surgery Done for Ritual Reasons 6,000 Years Ago – This article reveals that between 6,000 and 4,000 years ago, skilled surgeons in southwestern Russia cut holes the size of silver dollars or larger out of the backs of people’s skills for ritual reasons. Miniature, Beating Hearts Grown Using Stem Cells – Researchers describe an approach that combines features of engineered heart muscle and cardiospheres: micro-heart muscle arrays, in which elongated muscle fibers are formed in an easily fabricated template, with as few as 2,000 iPS-cardiomyocytes per individual tissue. Genome Editing of Lineage Determinants in Human Pluripotent Stem Cells Reveals Mechanisms of Pancreatic Development and Diabetes – Researchers not only verified conserved gene requirements between mice and humans but also revealed a number of previously unsuspected developmental mechanisms with implications for type 2 diabetes. Chemical Control of Grafted Human PSC-Derived Neurons in a Mouse Model of Parkinson's Disease – This article shows tunable rescue of motor function in a mouse model of Parkinson’s disease, following transplantation of human midbrain dopaminergic neurons differentiated from human pluripotent stem cells (hPSCs) engineered to express DREADDs (designer receptors exclusively activated by designer drug). Combined Inhibition of DNMT and HDAC Blocks the Tumorigenicity of Cancer Stem-Like Cells and Attenuates Mammary Tumor Growth – A combination of the DNMT inhibitor 5-azacytidine and the HDAC inhibitor butyrate markedly reduced cancer stem cell abundance and increased the overall survival in this mouse model. Scientists Uncover New Way to Grow Rare Life-Saving Blood Stem Cells – This article shows that overexpression of the RNA-binding protein Musashi-2 (MSI2) induces multiple pro-self-renewal phenotypes, including a 17-fold increase in short-term repopulating cells and a net 23-fold ex vivo expansion of long-term repopulating hematopoietic stem cells. Photo Reference: Courtesy of Dr. Arnold Kriegstein Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

Dr. Kateri Moore, an associate professor at Icahn School of Medicine, shares her pioneering work on hematopoietic stem cells and their therapeutic potentials. She discusses innovative techniques for reprogramming somatic cells into hematopoietic cells and their implications for cancer therapies. The conversation also touches on the dormant states of these stem cells and their activation during stress. Insights into the challenges and breakthroughs in this dynamic field highlight the emotional journey of scientific research, alongside reflections on their personal milestones.

Guest: Stem cell researcher Dr. Joe Zhou from Harvard discusses his work and latest paper in Cell Stem Cell. His research covers beta cells, insulin regulation and organoids. Resources and Links Climate Change to Affect Wine Quality – This article suggests that climate change has fundamentally altered the climatic drivers of early wine grape harvests in France, with possible ramifications for viticulture management and wine quality. Men in US Have Lower Life Expectancies than Other Countries – This article presents the three main reasons why men in US have lower life expectancies, which are: gun violence, drugs, and car accidents. How to Tell if a T-Rex Is Pregnant – Scientists can determine if Tyrannosaurus rex is female and pregnant by the presence of medullary bone. Lost Memories Retrieved in Mice with Alzheimer’s Like Symptoms – Scientists try to retrieve “forgotten” memories in mice engineered to have Alzheimer’s symptoms. US Gets a Failing Grade in Healthy Lifestyle Behavior – Only 2.7% of all adults had all four healthy lifestyle characteristics, which are: being sufficiently active, eating a healthy diet, being a nonsmoker, and having a recommended body fat percentage. Over the Counter Painkiller More Dangerous They You Think – This article describes the effects of taking non steroidal anti inflammatory drugs especially the risk of heart complications and bleeding. Making Sperm from Stem Cells – Researchers report complete in vitro meiosis from embryonic stem cell-derived primordial germ cells. Four Key Genes Govern Growth, Multiplication of Blood Stem Cells – This article shows cohesin as a major regulator of hematopoietic stem cells and illustrate the power of global RNAi screens to identify modifiers of cell fate. Hematopoietic Reprogramming In Vitro Informs In Vivo Identification of Hemogenic Precursors to Definitive Hematopoietic Stem Cells – This article presents how reprogramming reveals a phenotype for in vivo precursors to hemogenic endothelium, establishing that direct in vitro conversion informs developmental processes in vivo. A New CRISPR Advance – CRISPR interference system in induced pluripotent stem cells (iPSCs) provides a powerful platform to perform genome-scale screens in a wide range of iPSC-derived cell types, dissect developmental pathways, and model disease. Reprogrammed Stomach Tissue as a Renewable Source of Functional β Cells for Blood Glucose Regulation – Scientists show that reprogramming of antral stomach cells assembled into bioengineered mini-organs in vitro yielded transplantable units that also suppressed hyperglycemia in diabetic mice, highlighting the potential for development of engineered stomach tissues as a renewable source of functional β cells for glycemic control. Photo Reference: Courtesy of Dr. Joe Zhou Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

Guest: We bring on writer and researcher Dr. Aubrey de Grey to discuss his work on anti-aging.  We talk with Dr. de Grey on his latest findings and his ultimate goal of eliminating aging so we call can one day celebrate our 1000th birthday. Resources and Links Sex-Specific Mechanisms of Stress Susceptibility – According to this article, stress-related mental illnesses are twice as prevalent in women as in men. Global Divergence of the Human Follicle Mite Demodex folliculorum: Persistent Associations between Host Ancestry and Mite Lineages – Researchers found that hosts with different regional ancestries harbor distinct lineages of mites and that these associations can persist despite generations spent in a new geographic region. Pain Produces Memory Gain – This article reveals that physical pain can make memories last longer. Integrated, Multi-Cohort Analysis Identifies Conserved Transcriptional Signatures across Multiple Respiratory Viruses – Researchers performed two integrated, multi-cohort analyses of publicly available transcriptional data of viral infections. Human Parasites in the Roman World – This article presents the archeological evidence for parasites in the Roman era in order to demonstrate the species present at that time, and highlight the health consequences for people living under Roman rule. Key Elements of Healthy Eating Patterns – This article provides guidelines and key recommendations for individuals to enhance eating and physical activity patterns. 9 Science-Backed Steps to Have the Best Sleep of Your Life – Tonight – This article shares nine simple, science-backed strategies you can do today that'll increase your odds of sleeping better tonight and waking up refreshed tomorrow. StemCells (STEM) Names New CEO – StemCells Inc. announced that Dr. Ian Massey, its President and Chief Operating Officer, has been appointed by the Board of Directors to succeed Martin McGlynn as the company’s Chief Executive Officer. A Protein Biomarker Confirms the Presence of Stem Cells that Maintain Ovaries – Stem cell activity in the outer lining of the ovary, now identified in mice by A*STAR researchers, will elucidate normal ovarian activity and offer insights into the origins of disease. Defined Three-Dimensional Microenvironments Boost Induction of Pluripotency – Researchers find that the physical cell confinement imposed by the 3D microenvironment boosts reprogramming through an accelerated mesenchymal-to-epithelial transition and increased epigenetic remodeling. Engineered Stem Cells May Help in ALS – Stem cells engineered to secrete neurotrophic growth factors may slow progression of amyotrophic lateral sclerosis (ALS). Slow Stem Cell Division May Cause Small Brains – Duke University researchers have figured out how a developmental disease called microcephaly produces a much smaller brain than normal: some cells are simply too slow as they proceed through the neuron production process. Genetically Engineering Self-Organization of Human Pluripotent Stem Cells into a Liver Bud-Like Tissue Using Gata6 – Scientists present a novel approach for generating and then co-differentiating human induced pluripotent stem cell-derived progenitors through a genetically engineered pulse of GATA-binding protein 6 (GATA6) expression. Photo Reference: Courtesy of Dr. Aubrey de Grey Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

Guest: We feature some of our conversations with attendees from the 2015 World Stem Cell Summit from Atlanta, GA. Happy New Year! Resources and Links 4 New Elements Are Added to the Periodic Table – This article reports that the elements with atomic numbers 113, 115, 117 and 118 will get permanent names soon, according to the International Union of Pure and Applied Chemistry, with two of the four elements now known as ununseptium and ununtrium. New Type of Moon Rock Discovered by China's Yutu Lunar Rover – China's Yutu lunar rover made the discovery on a long-dead lava flow in the giant Mare Imbrium dark spot and found the volcanic rock known as basalt which has intermediate levels of titanium. CMV Vaccine Created at City of Hope Is Found Safe, Effective in Patients Receiving Stem Cell Transplants – City of Hope researchers report that an innovative vaccine known as CMVPepVax, that boosts cellular immunity against cytomegalovirus, or CMV, is safe and effective in stem cell transplant recipients. Melting Sea Ice Increases Arctic Precipitation, Complicates Climate Predictions – This article reports that the melting of sea ice will significantly increase Arctic precipitation, creating a climate feedback comparable to doubling global carbon dioxide, according to the findings of Dartmouth College-led study. Eating Poorly and Depressed? Yale Team Finds Connection — And Maybe a Solution – Yale researchers report that effects of a high-fat diet overlap with those of chronic stress and could also be a contributing factor in depression as well as metabolic disorders, such as type 2 diabetes. MERS Vaccine Protects Camels, Which Is Good for People – The vaccine, which was developed by German scientists, protects against the virus that causes Middle East respiratory syndrome (MERS) and has been shown to be effective in camels. Asperger's and Autism Linked to Decreased Action of Neurotransmitter GABA: Study – A new study from Harvard and MIT links autism symptoms to reduced action of GABA, a neurotransmitter responsible for dampening cellular activity in the brain. Is Extreme Longevity Genetic? Scientists Find Centenarians Lack Genes Underlying Certain Chronic Diseases – A group of U.S. and international researchers cited genetic studies have thus far identified a single gene, APOE, absent in centenarians, or the people who are one hundred or more years old. Stem Cells May Save Northern White Rhinos – This article reveals how researchers plan to use stem cells to create fertilized rhino embryos, which will be carried by surrogate southern white rhino females. Banking on Stem Cells at Cellular Dynamics International – Cellular Dynamics International is still one of the prominent companies at Unviersity Reasearch Park since it was purchased by Fujifilm, and plans to add staff in 2016. Human-Mouse Chimerism Validates Human Stem Cell Pluripotency – Researchers show, using a stage-matching approach, that human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells have the capacity to participate in normal mouse development when transplanted into gastrula-stage embryos, providing in vivo functional validation of hiPSC pluripotency. Grafted Human iPS Cell-Derived Oligodendrocyte Precursor Cells Contribute to Robust Remyelination of Demyelinated Axons after Spinal Cord Injury – This article describes how researchers took advantage of their recently developed protocol to obtain human-induced pluripotent stem (iPS) cell-derived oligodendrocyte precursor cell-enriched neural stem/progenitor cells and report the benefits of transplanting these cells in a spinal cord injury model. Identification of Drugs that Regulate Dermal Stem Cells and Enhance Skin Repair – Researchers identified and validated five compounds that has the ability to enhance the self-renewal of human and rodent skin-derived precursors, and showed that two of them, alprostadil and trimebutine maleate, enhanced the repair of full thickness skin wounds in middle-aged ...

Guest: Scott Dylla, CSO and co-founder of Stemcentrx, a really cool new company that is using novel technology to target stem cells as a therapy for different cancers, joins us for the interview portion. Happy Holidays and New Year! Photo Reference: Courtesy of Scott Dylla Subscribe to our newsletter! Never miss updates about new episodes. Subscribe