The Stem Cell Podcast

Ep. 76: “Glyco-Pure” Featuring Dr. Stephen Duncan

Oct 11, 2016
Dr. Stephen Duncan, a leading figure in regenerative medicine from the Medical University of South Carolina, explores the purification of cell lineages derived from pluripotent stem cells. He discusses the challenges of heterogeneity in iPSC differentiation and the significance of glycoproteomics in identifying hepatocyte-specific markers. Duncan also evaluates clinical applications for liver therapies, including innovative methods for scaling up cell purification and modeling rare diseases. His insights shed light on the future of liver regeneration and potential therapeutics.
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ANECDOTE

Lab Shift From Mice To Human iPSCs

  • Duncan's lab shifted from mouse embryonic models to human pluripotent stem cells to better model human liver disease.
  • The Yamanaka/Thomson advances enabled disease-specific iPSC modeling in his group.
INSIGHT

Heterogeneity Masks Subtle Disease Signals

  • iPSC differentiations yield heterogeneous populations that reduce sensitivity for detecting subtle disease phenotypes.
  • Homogenizing cells by surface markers improves comparability across patient-derived lines.
ADVICE

Map Surface N-Glycoproteins First

  • Use cell-surface N-linked glycoproteomics to map proteins on primary target cells and derive purification markers.
  • Validate enriched proteins and develop antibodies to purify mature, functional cell populations.
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