Bloody Minded Episode 13 - In the Lab: Dr Coombs? Who is DAT? | Direct Coombs Test
Aug 20, 2024
Discover the intriguing world of the Direct Antiglobulin Test, a crucial tool in diagnosing immune-mediated hemolysis. The hosts dive into its historical context and the science behind the test, making complex concepts accessible and entertaining. Learn about the meticulous laboratory processes and the significance of interpreting positive and negative results. They also discuss the complexities of immune system interactions and potential errors to watch out for. It’s a thrilling blend of hematology and storytelling that unravels the mysteries of red blood cell health!
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DAT detects immune components on red cells
- The Direct Antiglobulin Test (DAT) detects immune system components bound to red cells, such as antibodies or complement.
- This determines if the immune system is causing red cell destruction, crucial in hemolysis workup.
Proper washing and reagents usage
- Wash patient red cells at least four times in saline before testing to remove unbound antibodies.
- Use monospecific and polyspecific anti-IgG and anti-C3 reagents to identify what is attached to red cells.
Positive DAT doesn't always mean hemolysis
- A positive DAT does not always indicate hemolysis; some positive results occur without clinical hemolysis.
- Up to 10% of hospitalized patients may have incidental positive DAT without immune hemolysis.