Bloody Minded Episode 17 - Enough about DAT | Approach to DAT Negative Haemolysis
Sep 17, 2024
Dive into the intriguing world of DAT-negative hemolysis! The hosts explore the complex causes including membrane and enzyme disorders while sharing a mix of medical insights and humor. Learn about the structural quirks of red blood cells, from hereditary spherocytosis to rare hydration disorders. Discover the EMA assay for diagnosing membrane defects and unravel the mysteries of enzymatic disorders like G6PD deficiency. With engaging discussions and useful takeaways, this episode is packed with hematological revelations!
AI Snips
Chapters
Transcript
Episode notes
Understanding DAT-Negative Hemolysis
- DAT-negative hemolysis occurs when red cells break down but direct antiglobulin test (DAT) is negative.
- It can be categorized as intrinsic red cell defects or extrinsic non-immune destruction.
Membrane Defects and Hemolysis
- Membrane disorders of red cells cause shape abnormalities like spherocytes or elliptocytes.
- Hereditary spherocytosis is the most common membrane defect leading to DAT-negative hemolysis.
Using Osmotic Fragility Tests
- Use osmotic fragility tests to screen for membrane defects causing red cell fragility.
- Interpret abnormal results cautiously as many conditions affect cell fragility nonspecifically.