The Fellow on Call: The Heme/Onc Podcast Episode 002: Heme Path Series, Pt 1 - Flow Cytometry
Jan 19, 2022
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Page Triggered Heme-Path Panic
- Speaker 1 describes freezing up when a hematopathology fellow paged with complex test terms during first-year call.
- The experience motivated this episode series to demystify heme-path testing for trainees.
Flow Cytometry Reveals Single-Cell Phenotypes
- Flow cytometry measures cell surface markers to define a cell's phenotype on a cell-by-cell basis.
- Modern cytometers detect 11+ fluorescent colors enabling complex multi-marker phenotyping.
How Cytometers Differentiate Cells
- Forward scatter approximates cell size while side scatter reflects internal complexity.
- Fluorescent antibodies map specific CD proteins to detector channels for marker quantitation.