This Week in Virology

The TWiVodrome considers the intestinal tract as an alternative infection route for MERS coronavirus, and how reduced accumulation of defective viral RNAs might lead to severe influenza. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode HaHa influenza virus (mBio) MERS coronavirus intestinal transmission (Sci Adv) Simulated gastric and intestinal fluids Stomacher Defective genomes and severe influenza (PLoS Path) Image credit Letters read on TWiV 481 Weekly Science Picks Kathy - CD45.1 and CD45.2 mice may not be functionally equivalent Rich - Relative humidity versus dewpoint (video) Dickson - Underwater photography winners Alan - Photo of a single atom Vincent - Proposed Federal science budget cuts and The Blog is Dead Listener Pick Jess - Louis Tompkins Wright Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv

Scott Hensley joins the TWiVites to review the current influenza season and presence of the virus in exhaled breath of symptomatic cases. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guest: Scott Hensley Become a patron of TWiV! Links for this episode Weekly influenza surveillance (CDC) Influenza vaccination status (CDC) H3N2 glycosylation site and eggs (PNAS) Influenza virus in exhaled breath (PNAS) Geometric Defects and Icosahedral Viruses (Viruses) Image credit Letters read on TWiV 480 Weekly Science Picks Kathy - Flow Virometry Rich - Falcon Heavy Launch links: Flight diagram; Launch including booster landing; Tesla in space; Tesla orbit; Reusable booster failures; Booster size Dickson - Next generation of weapons Alan - Ed Yong's story on fixing gender imbalance in science reporting Vincent - CD4 Hunter iOS Android Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv

The TWiVome explores induction of antiviral responses by repeating patterns of capsids, and a fungus in the mosquito gut that aids dengue virus replication. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode Innate immune detection of capsid patterns (mBio) Mosquito gut fungus aids dengue virus replication (eLife) Nominate MicrobeTV for a Keck award Image credit Letters read on TWiV 479 Weekly Science Picks Kathy - Great Lakes, false color Rich - Times 360 Lego factory tour Dickson - 3D printed houses Alan - The new terms for trust in journalism Vincent - A Tale of Two Cultures and Why Science Blogging Still Matters Listener Picks Jolene - Immune Quest Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv

The TWiV team explains how infectious horsepox virus - likely the ancestor of smallpox vaccines - was recovered from chemically synthesized DNA fragments. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode ASV 2018: asv.org, asv2018.umd.edu Potential Zika virus related birth defects, USA (MMWR) Bordering on an outbreak (Tex Obs) Horsepox virus from synthesized DNA (PLoS One) Horsepox based smallpox vaccine (NEJM) Has horsepox become extinct? (Vet Rec) Evolutionary path of smallpox vaccines (Lancet Inf Dis) Equination (Vaccine) Image credit Letters read on TWiV 478 Weekly Science Picks Kathy - UM EEB photo contest Rich - Dino-Lite digital microscope and digital microscope camera Dickson - Sandford Photography Alan - A list of female virologists Vincent - Virology Lectures 2018 Listener Picks Andrew - Interferome Neva - The Untreatable by Gavin Francis Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv

The TWiVodrome explains how a gag-like protein from a retrotransposon forms virus-like particles that carry mRNA within vesicles across the synapse. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode ASV 2018: asv.org, asv2018.umd.edu Arc virus-like particles transport RNA across the synapse (mammalian, Drosophila) Chemical synapse (Wikipedia) 2 minute video on the neuron Image credit Letters read on TWiV 477 Weekly Science Picks Kathy - Ice cliffs form over the weekend Rich - The Courage To Invent: A NASA Roboticist Tells Her Story (Ayanna Howard) Dickson - 2017 Was One of the Hottest Years on Record Alan - Experimenter gender and replicability Vincent - Set Your Autocorrect to Change 'Guys' to 'Folks' Listener Picks Asal - 100 million chickens with avian flu Mark - Dr. Tatiana's Sex Advice to All Creation Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv

Nels joins the TWiV team to talk about his work on genomic accordions in vaccinia virus, hepatitis B virus in a 439 year old mummy, and viral induction of energy synthesis by a long noncoding RNA. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guest: Nels Elde Click arrow to play Download TWiV 476 (63 MB .mp3, 105 min) Subscribe (free): iTunes, RSS, email Become a patron of TWiV! Links for this episode ASV 2018: asv.org, asv2018.umd.edu TWiV is a must-listen (WaPo) More on poxvirus accordions (bioRxiv) HBV from a 439 year old mummy (PLoS Path) lncRNA promotes viral replication by inducing metabolism (Science) Making Data Visual Letters read on TWiV 476 Weekly Science Picks Nels - Everyday Evolution Kathy - Paper-fold an ellipse Rich - Oxford Nanopore Technologies: YouTube channel; General technology; DNA sequencing; Sequencing singularity Dickson - Spiders that look like pelicans Alan - Guessing pool for China Spacelab reentry Vincent - How to take a picture of the stealth bomber over the rose bowl Listener Picks Gretchen - The Bearded Lady Project Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv

In the first episode for 2018, the TWiV team reviews the amazing virology stories of 2017. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler Become a patron of TWiV! Links for this episode ASV 2018: asv.org, asv2018.umd.edu Virology stories of 2017 - in no particular order A virologist in space - TWiV 444 Cool things viruses do: Astrovirus capsids are enterotoxins TWiV 423; giant Klosneuviruses encode lots of stuff for translation TWiV 437; Giant Noumeaviruses quickly make the nucleus temporarily leaky TWiV 440 TWiV 438 Drs. TWiV go to Washington and the March for Science Unexpected stuff: TWiV 451 Expectorate the unexpected; TWiV 470 Just a passing phage; TWiV 456 Be careful of canons; TWiV 455 Pork and genes; TWiV 432 gamete fusion protein from viruses TWiV 443 On a leaf no one can hear you scream. The puppet master. TWiV 448 Mavis the structure maven Viruses are us: TWiV 472 Riddle of the skinks; TWiV 439 The purloined envelope; TWiV 434 Live long and pupate Antiviral RNA: TWiV 433 Poops viruses and worms; TWiV 449 The sound of non-silencing; TWiV 450 Ben tenOever and RNA out Viruses as tools: TWiV 461 Gotta trace them all Flaviviruses: TWiV 473, 471, 468; 458; 454; 451; 432; 430; 429; 426 NSF survey of earned doctorates Letters read on TWiV 475 Weekly Science Picks Kathy - Voyager recording and book Rich - David Sheldrick Wildlife Trust (Maktao) Dickson - Global Croplands Map Alan - The Great Influenza one day at a time Vincent - The Doomsday Machine by Daniel Ellsberg Listener Picks Mark - Science Moms and Science of HIV Andy - Ask Me Anything Steve - Critical Attitude in Medicine Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv

The TWiVanguardians take on Bodo saltans virus, a leviathan which infects an abundant flagellated eukaryote in Earth's waters. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, and Kathy Spindler Become a patron of TWiV! Links for this episode ASV 2018: asv.org, asv2018.umd.edu Support Microbe.tv Anti-dengue antibody and disease (J Inf Dis) Bodo saltans virus (biorXiv) Kinetoplast DNA (Euk Cell) Crossword puzzle for book contest (pdf) Letters read on TWiV 474 Weekly Science Picks Kathy - Every second Dickson - Top 10 Science Images 2017 Alan - Shortwave Radiogram Vincent - Science March on Instagram Listener Picks Islam - Class Crossword (Armored Penguin) Peter - The Last of the Iron Lungs Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv

The Fellowship of the Virus discuss enhancement of dengue disease in humans: the contribution of antibody concentration and increased binding to Fc receptors. Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Become a patron of TWiV! Links for this episode ASV 2018: asv.org, asv2018.umd.edu Support Microbe.tv NEIDL is approved NIH lifts GOF moratorium (Science) Blackburn steps down as head of Salk Institute (SD Union Trib) ADE of dengue disease in humans (Science) Commentary on ADE of dengue disease (Science) ADE of dengue disease and antibody binding to Fc (Science) Letters read on TWiV 473 Weekly Science Picks Kathy - Today in Science History Dickson - 10 Breakthrough Technologies 2017 Rich - SpaceX to orbit a Tesla Alan - Debt in America Vincent - The Death of Expertise by Tom Nichols (NYTimes review) Listener Picks Stephen - A Concise Paper Johnye - Holiday song parodies (one, two, three) Tom - SMBC: Are viruses alive? Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv

Vincent speaks with professional podcast producer Ray Ortega about his career and the power of the podcast. Host: Vincent Racaniello Guest: Ray Ortega Become a patron of TWiV! Links for this episode Ray on Twitter The Podcasters' Studio Produce Picker Podcast Ray's YouTube channel Intro music is by Ronald Jenkees. Send your virology questions and comments to twiv@microbe.tv