Bloody Minded Episode 28 - In the Lab: Flow Cytometry, Mass Spectrometry & Cytogenetics in MM
Dec 17, 2024
Dive into the fascinating world of advanced diagnostic techniques like flow cytometry and mass spectrometry, especially in the context of multiple myeloma. Discover how flow cytometry deciphers cell characteristics with lasers and fluorescent tags. The importance of quality control in labs unfolds alongside the wizardry of mass spectrometry for analyzing paraproteins. Explore cytogenetic concepts like karyotyping and chromosomal abnormalities, revealing their crucial roles in prognosis. Science and humor collide, making complex topics accessible and engaging!
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Basics of Flow Cytometry
- Flow cytometry rapidly measures multiple characteristics of cells using fluorescent markers and lasers.
- It identifies cell size, complexity, and antigen profiles to distinguish cell types and clonality.
Cell Size and Complexity in Flow
- Forward scatter in flow cytometry measures cell size by light bending around an obscuration bar.
- Side scatter measures cell complexity, aiding in separating different cell populations.
Use Euroflow Guidelines
- Use Euroflow consortium guidelines for standardized sample preparation, antibody panels, and data analysis in flow cytometry.
- These guidelines optimize identification of small cell populations and minimal residual disease detection.