The Stem Cell Podcast

Ep. 309: “Neuronal Cell Fate and Function” Featuring Dr. Thomas Vierbuchen

Nov 25, 2025
Guest
Thomas Vierbuchen
Dr. Thomas Vierbuchen, an Assistant Professor at Memorial Sloan Kettering Cancer Center, explores the intricacies of neural development through pluripotent stem cells. He discusses the advantages of 2D cultures versus complex organoids for research reproducibility. Vierbuchen recounts the surprising journey of direct reprogramming from fibroblasts to neurons and the challenges faced in translating these techniques to human cells. He also highlights the importance of understanding embryogenesis and the intriguing question of how organs determine their size.
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INSIGHT

Genome Controls Brain Cell Diversity

  • Vierbuchen's lab uses mouse and human PSCs to model how the genome orchestrates brain development and neuronal diversity.
  • They focus on directed differentiation and organoids to study gene regulation across species and individuals.
ADVICE

Prefer 2D For Scalable Screens

  • Use 2D models for scalable, reproducible phenotypic screens and simpler molecular studies.
  • Prefer organoids only when complexity is required, since they are harder to generate and analyze reproducibly.
ANECDOTE

Discovery In A Whirlwind Year

  • Vierbuchen recounts joining Marius Wernig's lab and discovering direct neuronal conversion while testing proneural factors on fibroblasts.
  • The initial excitement mixed with stress as they validated the robust neuronal phenotype and addressed reproducibility concerns.
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