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Freely Filtered 042: Nephrin Antibodies Cause Minimal Change Disease. Wait...What?
Freely Filtered, a NephJC Podcast
Immunoprecipitation for Nephron Staining
We wanted to see if there's a bind, there's a, there's binding of the antinephrine antibodies to the nephron. We basically incubated the patient serum with human glomerular extract from a healthy kidney. And then we took that and we ran it over protein G beads,. So all the immunoglobulins would bind to those beads. Then you elute that out and then you run that alluate on a western blot and then incubate with an antinephrine antibody to see if you can get a band. But what we ended up getting when we incubated that for long enough time to see the nephrons band, we got a lot of
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