How to Improve the Nitrogen Content in Your Yeast Extracts?

In the paper, we define that we need 100 carbon to nitrogen. So if you know what the sugar concentration of your medium is, you should be able to determine how much nitrogen you need. Depending on the yeast extract source that you procure, depending on the supplier, there may be varying concentrations of nitrogen. You can use that calculation based on what you need for your medium.