Freely Filtered 042: Nephrin Antibodies Cause Minimal Change Disease. Wait...What?
Freely Filtered, a NephJC Podcast
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Using Confocal Microscopy to Detect Antigens
The IGG is recognized by an anti human IGG antibody that has a fluorophore bound to it directly. When you use these confocal microscopes, they all this microscopy, how are you seeing the antibodies that these are because you've already labeled you? Yeah. It's not necessary. If you have a really good confocal microscope, you probably can see what we saw. And we did see it with confocal microscope. But it's just even better and even prettier. You can make this z stack of pictures. So there's the x axis, the y axis and then the z is kind of like, you know, the depth of the tissue. And you
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