Research suggests that maternal hormone levels, particularly testosterone, may influence sexual orientation. There is a correlation between the number of older brothers and the likelihood of being gay, potentially due to pregnant individuals building anti-male factors from carrying male pregnancies. This could result in younger brothers being exposed to anti-male antibodies, which may prevent typical testosterone masculinization. Furthermore, studies on finger digit ratios indicate that testosterone exposure in the womb alters physical traits in homosexuals compared to heterosexual peers. This points to a complex interaction between hormones and sexual attraction, illustrating that testosterone setting influences may work differently across sexual orientations.

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