This Week in Virology

Hosts: Vincent Racaniello, Alan Dove, Rich Condit, and Kathy Spindler The sages of TWiV explain how chronic wasting disease of cervids could be caused by spontaneous misfolding of prion protein, and the role of the membrane protein Axl in Zika virus entry into cells. Become a patron of TWiV! Links for this episode Register for ASV 2017 ASM 2017 election is now open - VOTE HERE ASM Election questions: Which positions are we voting for? What is the ASM Board of Directors? Organization Chart What is the Council on Microbial Sciences? Organization Chart FAQs Scientists March on Washington Spontaneous generation of unique cervid strain (mSphere) Zika virus entry into human glial cells via Axl (Cell Rep) Article collections: PNAS, Cell, Nature, Virology Letters read on TWiV 426 This episode is brought to you by Blue Apron. Blue Apron is the #1 fresh ingredient and recipe delivery service in the country. See what's on the menu this week and get your first 3 meals free – WITH FREE SHIPPING – by going to blueapron.com/twiv. Weekly Science Picks Alan - Deerland by Al Cambronne Dickson - Antarctica's Colorful Underwater World Kathy - You can't unring a bell #1 and #2 Rich - Extra letters added to life's genetic code (source) and The Doomsday Clock (wiki) Vincent - Trump Science Advisors, National Parks Won't Be Silenced Listener Pick Stefan - The Vital Question by Nick Lane Kevin - Scientists March on Washington? Dennis - Fractal Tree of Life Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The TWiVaniellos discuss a thermostable poliovirus empty capsid vaccine, and two cell genes that act as a switch between entry and clearance of picornavirus infection. Become a patron of TWiV! Links for this episode Register for ASV 2017 ASM-ASV Conference on Interplay of Viral and Bacterial Pathogens XKCD #242 Thermostable poliovirus empty capsid vaccine (PLoS Path) Switch between viral entry and clearance (Nature) Glass poliovirus by Jason Roberts Letters read on TWiV 425 This episode is brought to you by Blue Apron. Blue Apron is the #1 fresh ingredient and recipe delivery service in the country. See what's on the menu this week and get your first 3 meals free – WITH FREE SHIPPING – by going to blueapron.com/twiv. Weekly Science Picks Alan - Medical lab whirligig Dickson - Antarctica's Colorful Underwater World Kathy - Prince Rupert's drops #1 and #2 Rich - Dylan's 30th - Eric Clapton --Don't Think Twice, It's All Right (The 30th Anniversary Concert Celebration) Vincent - Saving climate data and Public Good and Public Funding of Science Listener Pick Suellen - For the anti-vaccine people Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guest: Trudy Rey Trudy joins the the TWiVlords to discuss new tests for detecting prions in the blood, and evidence showing that foamy retroviruses originated in the seas with their jawed vertebrate hosts at least 450 million years ago. Become a patron of TWiV! Links for this episode Welcome omega tau to MicrobeTV Register for ASV 2017 Oliver Smithies dies Assays for prions in the blood (one, two) Biosafety working on prions (Michigan State) Marine origin of retroviruses in Palaeozoic era (Nature Comm) Cancer Virus by Dorothy Crawford Adenovirus and obesity (Int J Chronic Dis) Image credit Letters read on TWiV 424 Weekly Science Picks Trudy - Artist Jenni Viljaniemi Alan - Radio Garden Dickson - World's First Green Energy Boat Kathy - mSphereDirect video and web Rich - Deathworld Trilogy by Harry Harrison Vincent - Trump's Vaccine Panel (NYTimes), Offit's Response (Daily Beast), ASM Letter Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guest: Jared Rice The TWiV academia discuss induction of diarrhea by the capsid protein of an astrovirus, and association of a fungal RNA virus with white-nose syndrome of North American bats. Become a patron of TWiV! Links for this episode Register for ASV 2017 ASM-ASV Conference on Interplay of Viral and Bacterial Pathogens Astrovirus capsid protein induces diarrhea (mBio) Fungal virus associated with white-nose syndrome (PLoS Path) Image credit Letters read on TWiV 423 Weekly Science Picks Alan - GPS visualizer Dickson - James Webb Space Telescope and The Vertical Farm Kathy - First Snowflake Photos (One and Two) Rich - Red Mars by Kim Stanley Robinson Vincent - Carrie Fisher Fought for the Rebel Alliance and Mental Health Awareness Listener Pick Ken - The Abate Lab Sean - The Death of Expertise Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The TWiVestigators wrap up 2016 with a discussion of the year's ten compelling virology stories. Become a patron of TWiV! Should auld infections be forgotAnd never T-cells primed?Will cross-reacting antigensProtect the host next time? Links for this episode Register for ASV 2017 ASM-ASV Conference on Interplay of Viral and Bacterial Pathogens Ten virology stories from 2016 Zika virus (first covered in 2015, TWiV 368) Ebolavirus Eukaryotic genes in Wolbachia phage (TWiV 412) Harold "400" Varmus (TWiV 400) and many other guests Vaccines Mutualism Mosquitoes and viruses Vector victorious (TWiV 401) Animal models of virus infections Virus evolution Current Zika virus infections (ECDC) Early Zika virus infections in Hawaii (PLoS NTD) Zika virus vaccine for whom? (NYTimes) Final results for Ebola virus vaccine (Lancet) Photo from Mohammad Letters read on TWiV 422 This episode is brought to you by CuriosityStream, a subscription streaming service that offers over 1,400 documentaries and non­fiction series from the world's best filmmakers. Get unlimited access starting at just $2.99 a month, and for our audience, the first two months are completely free if you sign up at curiositystream.com/microbe and use the promo code MICROBE. Weekly Science Picks Alan - North America's Lost Medieval City Dickson - New Views of Pillars of Creation Kathy - Reilly Top Ten Rich - Everyday Einstein Vincent - Naples' Sleeping Volcano Might Be Waking Up Listener Pick John - RadioLab: The Times They Are a-Changin' Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler Guest: Nathan Letts The TWiVnauts present another example of an infectious but replication incompetent vaccine, an insect specific arborvirus bearing chikungunya virus structural proteins. Become a patron of TWiV! Links for this episode Register for ASV 2017 ASM-ASV Conference on Interplay of Viral and Bacterial Pathogens Chikungunya virus vaccine using insect virus platform (Nat Med) Ebola vaccine results (NYTimes, paper) Letters read on TWiV 421 This episode is brought to you by CuriosityStream, a subscription streaming service that offers over 1,400 documentaries and non­fiction series from the world's best filmmakers. Get unlimited access starting at just $2.99 a month, and for our audience, the first two months are completely free if you sign up at curiositystream.com/microbe and use the promo code MICROBE. Weekly Science Picks Alan - Open Access Button Dickson - National Geographic Photographs Kathy - World's Tiniest Radio Receiver Nathan - Mutter Museum, Philadelphia Vincent - How Rogue One is Connected to the Mac Listener Pick Sam - Cell Lab: Evolution Sandbox Hannelore - Congressional Dish Johnye - The Fungi in Your Future Brendan - Zimmer on Longform podcast Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The TWiV gurus describe how to use an orthogonal translation system to produce infectious but replication-incompetent influenza vaccines. Become a patron of TWiV! Links for this episode Register for ASV 2017 Local Zika virus transmission in Texas Texaszika.org Local dengue virus transmission in Texas (EID) No more Zika virus transmission in Florida (FL DOH) Dengue virus antibodies enhance Zika virus infection (Clin Trans Imm) Infectious, replication incompetent influenza virus vaccine (Science) Pyrrolysl-tRNA synthetase (Biochem Biophys Acta) History of nonsense suppression (Stan Maloy) Suppression of poliovirus amber mutant (Cell) Remembering mumps (PLoS Path) Letters read on TWiV 420 This episode is brought to you by CuriosityStream, a subscription streaming service that offers over 1,400 documentaries and non­fiction series from the world's best filmmakers. Get unlimited access starting at just $2.99 a month, and for our audience, the first two months are completely free if you sign up at curiositystream.com/microbe and use the promo code MICROBE. Weekly Science Picks Alan - Superbugs game Dickson - Dinosaur tail trapped in amber Rich - Earth's Rotation is Slowing (original article)Kathy - Lithium Ion batteries (one and two) Vincent - Truth and Lies in the Age of Trump (virology blog) and self driving Uber car Listener Pick Steve - New York City Street Tree Map Justin - Transmissible vaccines? Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The TWiVrific gang reveal how integration of a virophage into the nuclear genome of a marine protozoan enhances host survival after infection with a giant virus. Become a patron of TWiV! Links for this episode Microcephaly associated with Zika virus infection in Colombia (MMWR) Virophage genome integration and activation by superinfection (Nature) Don Nelson interview (pdf) Page view and h index for paper rank (PLoS One) Credit for image of C. roenbergensis Letters read on TWiV 419 This episode is brought to you by CuriosityStream, a subscription streaming service that offers over 1,400 documentaries and non­fiction series from the world's best filmmakers. Get unlimited access starting at just $2.99 a month, and for our audience, the first two months are completely free if you sign up at curiositystream.com/microbe and use the promo code MICROBE. Weekly Science Picks Alan - Lab Wars Dickson - Star in a Jar Fusion Reactor Rich -You're a Bee. This is What it Feels Like.Kathy - List of Potential Predatory Journals Vincent - CRISPR Patent Trial Begins Listener Pick Drake - Spinzall Katreya - Periodic Videos Pritesh - Smallpox in mummy Send your virology questions and comments to twiv@microbe.tv

Hosts: Vincent Racaniello, Dickson Despommier, Alan Dove, Rich Condit, and Kathy Spindler The TWiVsters describe a new animal model for MERS coronavirus-induced acute respiratory distress syndrome, produced by CRISPR/Cas9 editing of the mouse gene encoding an ortholog of the virus receptor. Become a patron of TWiV! Links for this episode New mouse model for MERS (Nat Micro) RNA-guided genome engineering (Science) Photo by Finny Letters read on TWiV 418 This episode is brought to you by CuriosityStream, a subscription streaming service that offers over 1,400 documentaries and non­fiction series from the world's best filmmakers. Get unlimited access starting at just $2.99 a month, and for our audience, the first two months are completely free if you sign up at curiositystream.com/microbe and use the promo code MICROBE. 0:25, 27:50 This show is sponsored by Drobo, a family of safe, expandable, yet simple to use storage arrays. Drobos are designed to protect your important data forever. This Holiday season give someone a Drobo to keep all their files and memories safe forever. TWiV listeners can save 20% or more off of their purchase of a Drobo 5D, Drobo 5Dt, Drobo 5N, or any 8-drive or 12-drive system at www.drobostore.com by December 31, 2016 using discount code MICROBE20. Register for ASV 2017 at Madison, Wisconsin Weekly Science Picks Alan - Human population through time Dickson - Every Orbit Cassini Has Taken Rich - OK GO: Walk Her WalkKathy - Medieval Bestiary Online Vincent - Can You Out-Science an Eighth Grader? and Stitchen Pox Listener Pick Hannah - Bioart and Bacteria Send your virology questions and comments to twiv@microbe.tv

Host: Vincent Racaniello Guest: Gary Nabel Vincent speaks with Gary Nabel, Chief Scientific Officer at Sanofi and former Director of the Vaccine Research Institute of NIAID, about his career and his work on HIV vaccines. Become a patron of TWiV! Links for this episode World AIDS Day Video of this interview (YouTube) Send your virology questions and comments to twiv@microbe.tv