The Stem Cell Podcast

Guest: Dr. Emma Hammarlund is an Associate Professor at the Division of Translational Cancer Research at Lund University. Her lab investigates how the rise of animals on Earth relates to the evolution of stemness control. They use both geobiology and tumor biology to explore the evolutionary roots of animal health and disease. Featured Products and Resources: Keep Current With the Latest in ESC and iPSC Research Try the New Standard in hPSC Maintenance: mTeSR™ Plus Medium The Stem Cell Science Round Up Cardioids That Beat – Scientists generated self-organizing cardiac organoids with hollow chambers that can contract rhythmically, like a heartbeat. Fluorescence Imaging with Low Phototoxicity – Researchers used high-speed, high-resolution 3D imaging to observe inter- and intracellular interactions on a millisecond scale. An Atlas Charting Human Development – Scientists generated a multi-organ, single-cell transcriptome atlas and used it to compare organ and organoid development. Rescuing Neurogenesis in Alzheimer’s– Researchers found that increasing miR-132, a microRNA downregulated in Alzheimer’s, restored neurogenesis and memory deficits in a mouse model. Photo Reference: Courtesy of Dr. Emma Hammarlund Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

Guest: Dr. Themasap Khan is the Vice President at Civilization Ventures, a venture capital firm that invests in genomics, diagnostics, digital health, AI, and personalized and regenerative medicine.  He leads the firm’s scientific due diligence and academic outreach programs. Dr. Khan earned his in PhD in stem cell biology and regenerative medicine in Dr. Sergiu Pasca’s lab at Stanford, where his research focused on genome engineering, cerebellar organoid models, and live imaging to elucidate biological mechanisms underlying neuropsychiatric disorders. Featured Products and Resources: Win Your Conference Registration Fee for ISSCR 2021 Are Your Pluripotent Stem Cells What You Think They Are? Explore Now The Stem Cell Science Round Up Gene Therapy for Adenosine Deaminase (ADA) Deficiency – Scientists treated patients with ADA severe combined immunodeficiency with a lentiviral vector encoding human ADA, which led to high overall and event-free survival. Lineage Tracing through Somatic Mutations – Researchers used somatic mutations in hematopoietic progenitors from human fetuses to study the divergence of embryonic and extra-embryonic tissues during development. A Small Molecule Cocktail to Enhance Cell Survival – Scientists used high-throughput screening to identify a cocktail that improves the viability of hPSCs and differentiated cells by blocking several stress mechanisms. 2-Photon Live Imaging of Single Corneal Stem Cells  – Researchers identified discrete, functionally diverse stem cell niche compartments in the corneal limbus in the eyes of live mice. Photo Reference: Courtesy of Dr. Themasap Khan Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

Guest: Dr. Johan Jakobsson is a Professor of Molecular Neurogenetics at the Lund Stem Cell Center. His research focuses on epigenetic mechanisms of the brain, including the role of microRNAs, long non-coding RNAs, and endogenous retroelements in neural stem cells and neurons. Featured Products and Resources: Win Your Conference Registration Fee for ISSCR 2021 Access Resources for Your Neuroscience Research The Stem Cell Science Round Up Intestinal Organoids to Model Self-Organization – Researchers found that stem cell zones in intestinal organoid cultures are shaped by fission events, and that organoid inflation drives stem cell differentiation. Wound Healing Without Scarring – Preventing Engrailed-1 activation during wound healing promotes skin regeneration with recovery of skin appendages, ultrastructure, and mechanical strength. Differentiation Unmasks Hidden Aspects of Aging in Stem Cells – Scientists used single-cell RNA sequencing to study age-related changes in muscle stem cells and fibro-adipose progenitors, and found an energy barrier in myogenic differentiation. Human-Monkey Chimeric Embryos  – Researchers demonstrated that hEPSCs survived, proliferated, and generated several peri- and early post-implantation cell lineages inside monkey embryos. Photo Reference: Courtesy of Dr. Johan Jakobsson Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

Guest: Dr. Marinna Madrid is Co-Founder of Cellino, an early-stage biotechnology company seeking to make personalized, autologous cell therapies viable at large scale for the first time. Cellino’s platform combines label-free imaging and high-speed laser editing with machine learning to automate cell reprogramming, expansion, and differentiation in a closed cassette format, enabling thousands of patient samples to be processed in parallel in a single facility. Featured Products and Resources: Keep current with the latest in cell therapy research with Cell Therapy News. Show the world what stem cell research looks like from your perspective by taking part in this year’s #StemCellfie contest. The Stem Cell Science Round Up Using IGF1 to Rescue HSCs from the Hallmarks of Aging – Researchers found that a decline in IGF1 in the bone marrow microenvironment initiates hematopoietic stem cell aging, and that direct stimulation with IGF1 could reverse this phenotype. Krüppel-Like Factor 1 is Critical for Heart Regeneration in Zebrafish – Krüppel-Like Factor 1 stimulates new cardiomyocyte production by reprogramming gene networks that regulate cardiomyocyte differentiation and mitochondrial metabolism. How Aneuploid Embryos Can Lead to Healthy Births – Scientists showed that mosaic embryos can be rescued through BMP4-dependent apoptosis of aneuploidy in the primary germ layers. Patient-Derived Cervical Organoids  – Generated from a pap smear sample, cervical organoids can be used as an experimental platform for sexually transmitted infection and cervical cancer research. Photo Reference: Courtesy of Dr. Marinna Madrid Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

Dr. Brenda Raud, a postdoctoral researcher focusing on T cell immunology, and Dr. Jason Goldsmith, a physician-scientist specializing in gut immunity, discuss their new Immunology Podcast. They delve into T-cell therapies for solid tumors, the impact of the microbiome on immunity and cancer treatment, and the importance of diet and obesity in immune health. They also share how COVID-19 has changed public perceptions of immunology and their motivation for launching the podcast to simplify complex research for a broader audience.

Guest: Dr. Krishanu Saha is an Associate Professor in the Department of Biomedical Engineering at the University of Wisconsin-Madison. His lab utilizes quantitative and bioengineering methods including genome editing, biomanufacturing, and disease modeling to advance the next generation of cell and gene therapies. Featured Products and Resources: Subscribe to ESC & iPSC News Survey Report: Hurdles of Genome Editing Using CRISPR-Cas9 The Stem Cell Science Round Up Growing Advanced Embryos Outside the Uterus – Researchers removed mouse embryos from the uterus at five days of gestation and grew them for six more days in roller culture systems, allowing them to reach advanced organogenesis. Lacrimal Gland Organoids That “Cry” – Scientists established long-term 3D organoid cultures of human and mouse lacrimal glands and used neurotransmitters to induce tear secretion. Generating Human “Blastoids” – Starting with either human ESCs or iPSCs, researchers used a three-dimensional culture strategy to generate blastocyst-like structures in vitro. Reprogramming Fibroblasts into iBlastoids – Fibroblasts were reprogrammed in vitro into three-dimensional models of the human blastocyst, termed iBlastoids. SARS-CoV-2-Induced Cardiac Damage in iPSC-Derived Heart Cells  – Scientists infected iPSC-derived cardiac cells with SARS-CoV-2 to better understand how the virus attacks the heart. Cytokine Storms in Cardiac Organoids – Using SARS-CoV-2-infected cardiac organoids and mice, researchers found that bromodomain and extraterminal domain family inhibition reduced infection and cardiac damage. Photo Reference: Courtesy of Dr. Krishanu Saha Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

Guest: Dr. Kelly Shepard is the Associate Director of Discovery and Translation at the California Institute for Regenerative Medicine (CIRM). CIRM was created by the voters of California in 2004. The agency funds stem cell research at institutions and companies throughout California with the goal of accelerating treatments to patients with unmet medical needs. Featured Products and Resources: Contest: Subscribe to Organoid News for a Chance to Win a Personalized Lab Coat Try mTeSR™ Plus The Stem Cell Science Round Up Intestinal Organoids for Regeneration – Researchers have generated a functional small intestinalized colon using ileum-derived organoids, offering a possible strategy for short bowel syndrome treatment. Gene Disrupts Lipid Homeostasis in Glia – Scientists have shown that APOE4 has widespread effects on the brain’s ability to metabolize lipids and respond to stress, offering an explanation for why the gene enhances Alzheimer’s risk. Maturation of Year-Old Brain Organoids – 3D cultures of human brain cells kept alive for nearly a year have been shown to undergo transitions in gene activity that resemble those seen in newborns. Brain Cell Grafts Reverse Parkinson’s Symptoms – Grafting neurons grown from a monkey’s own cells into their brain relieved the debilitating movement and depression symptoms associated with Parkinson’s disease. Photo Reference: Courtesy of Dr. Kelly Shepard Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

Guest: Dr. Willy Lensch is Strategic Advisor to the Dean at Harvard Medical School. Previously Executive Director of the Harvard Department of Stem Cell and Regenerative Biology, his experience in research, education, administration, science policy, intellectual property, science consulting, and outreach have combined to support and advance stem cell science and discovery at Harvard. Featured Products and Resources: Contest: Subscribe to Organoid News for a Chance to Win a Personalized Lab Coat STEMCELL Technologies Survey Report: Where Is Pluripotent Stem Cell Research Now? The Stem Cell Science Round Up Human Heart-Forming Organoids – For the first time, researchers have reproduced early human heart development in a culture dish using human pluripotent stem cells. Bile Duct Organoids Used to Repair Human Livers – A new study has found that when transplanted into damaged mouse or human livers, cholangiocyte organoids functioned normally and repaired bile ducts. Reversing Severe Muscle Wasting in Disease, Aging, and Trauma – Scientists have discovered a novel protein that triggers muscle stem cells to regenerate, leading to complete muscle replacement and movement in mouse models. Deletion of Tumor Suppressor Gene Enhances HSC Self-Renewal – Deletions of the Kmt2c gene selectively protect cycling hematopoietic stem cells (HSCs) from differentiation without inducing HSC proliferation themselves. Photo Reference: Courtesy of Dr. Willy Lensch Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

Guest: Dr. Sean Wu is an Associate Professor of Medicine at the Stanford School of Medicine. The Wu lab seeks to identify mechanisms responsible for human congenital heart disease, and uses mouse models and stem cells to study cardiovascular developmental biology, and to engineer cardiac tissue. Featured Products and Resources: Webinar: Modeling Arrhythmias Using hPSC-Derived Cardiomyocytes and Tracking Their Excitability Try MyoCult™ Myogenic Progenitor Culture Media and Supplements The Stem Cell Science Round Up hESC-Derived Dopamine Progenitors for Parkinson’s Treatment– Researchers have generated midbrain dopamine neurons from hESCs, and manufactured large-scale cryopreserved dopamine progenitors for clinical use in the treatment of Parkinson’s disease. Reactivating Neural Crest Pluripotency – A new study suggests that cranial neural crest cells expand their developmental potential through a transient reacquisition of molecular signatures of pluripotency. Engaging Eosinophils against Liver Injury – Scientists have discovered a mechanism of eosinophil-mediated liver protection that could serve as a therapeutic target to improve outcomes of patients undergoing liver transplantation. Disrupting the Identity of Heart Muscle Cells – Mutations in the LMNA gene severely disrupt chromatin organization in cardiomyocytes, but not hepatocytes or adipocytes, leading to abnormal activation of non-heart muscle genes. Photo Reference: Courtesy of Dr. Sean Wu Subscribe to our newsletter! Never miss updates about new episodes. Subscribe

Guest: Dr. Jürgen Knoblich is Scientific Director of the Institute of Molecular Biotechnology at the Austrian Academy of Sciences. The Knoblich lab is known for the development of an organoid model of early brain development, and is currently using iPSCs and cerebral organoids to investigate inter-brain region interactions, neurodevelopmental disorders, and neuronal connections and functions. Featured Products and Resources: Webinar: Challenges in Translating iPSC Technology Try the STEMdiff™ Cerebral Organoid Kit The Stem Cell Science Round Up Study Reveals Immune Driver of Brain Aging – Researchers have identified a key factor in mental aging, and suggested that it might be prevented or reversed by reprogramming myeloid glucose metabolism. A Tonsil Organoid System for Studying Adaptive Immunity – Scientists have developed a tonsil organoid system, and used it to evaluate immune responses to rabies and SARS-CoV-2 vaccines. Antisense Oligonucleotide Targets IRF4 Gene to Treat Multiple Myeloma – Researchers have shown that silencing IRF4 with an antisense oligonucleotide impairs myeloma cell survival and promotes sensitivity to clinical drugs. Illuminating the Path to Cervical Cancers – By creating novel organoid models, scientists have established a new approach to studying the biology of the cervix, and have identified key turning points in cancer development. Photo Reference: Courtesy of Dr. Jürgen Knoblich Subscribe to our newsletter! Never miss updates about new episodes. Subscribe