GasGasGas - The FRCA Primary Exam Podcast Gas Gas Gas - Michaelis Menten Enzymes!
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Oct 25, 2024 Dive into the fascinating world of enzyme kinetics! Learn about the roles of competitive and non-competitive inhibitors and how they impact enzyme activity. Discover the nuances of allosteric regulation and the significance of cofactors. The podcast breaks down the Michaelis-Menten equation and its graphical interpretations, including the effects of different kinetics. Clinical examples like phenytoin and alcohol highlight practical implications. Tune in for a deep understanding of enzyme behavior in the human body!
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Enzymes And The Law Of Mass Action
- Enzymes are protein catalysts that lower activation energy and can drive bi-directional reactions.
- The law of mass action links reaction velocity to reactant concentrations and defines an equilibrium constant, k.
Michaelis-Menten Key Parameters
- The Michaelis-Menten equation models enzyme reaction velocity using Vmax, Km, and substrate concentration [S].
- Km equals the substrate concentration that achieves 50% of Vmax, analogous to an ED50 concept.
Visualise The Michaelis-Menten Curve
- When drawing or memorising kinetics, visualise the hyperbolic curve that rises steeply then plateaus as enzymes become saturated.
- Remember a low Km means high enzyme affinity and thus half-maximal velocity at low substrate concentration.