The Stem Cell Podcast

Ep. 67: “Blood Stem Cell Therapy” Featuring Dr. Kristin Hope

Jun 7, 2016
Dr. Kristin Hope, a stem cell researcher and Assistant Professor, shares her groundbreaking insights on umbilical cord blood-derived hematopoietic stem cells. She discusses her team's findings on Musashi-2, an RNA-binding protein that enhances stem cell self-renewal and expansion. Dive into the therapeutic potential of targeting Musashi-2 in leukemia treatments and the challenges and advantages of using cord blood for transplants. Kristin also highlights the need to explore understudied RNA-binding proteins in hematopoiesis, paving the way for future innovations.
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INSIGHT

Cord Blood HSCs: Promise and Limitation

  • Umbilical cord blood HSCs are clinically advantageous but limited by low cell numbers in each unit.
  • Understanding molecular regulators is key to expanding cord blood HSCs for adult transplants.
ANECDOTE

Experimental Strategy Recap

  • Dr. Hope explained the lab's strategy: combine loss- and gain-of-function experiments to probe MSI2.
  • They used RNAi knockdown, overexpression, RNA-seq and functional xenotransplants.
INSIGHT

Musashi-2 Drives HSC Self-Renewal

  • Musashi-2 (MSI2) emerged as a powerful regulator of human cord blood HSC self-renewal.
  • MSI2 overexpression drove ex vivo expansion and a stem-cell-like expression signature.
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