The Stem Cell Podcast

Ep. 307: “Vascular Biology” Featuring Dr. Juan Melero-Martin

Oct 28, 2025
Dr. Juan Melero-Martin, an Associate Professor and Endowed Chair in Cardiac Surgery at Boston Children's Hospital, dives into vascular biology and regenerative medicine. He discusses groundbreaking findings on mitochondrial transfer from MSCs to endothelial cells and its role in enhancing cell function. Juan explains strategies for generating vascular organoids and the importance of transcription factors like ETV2 in differentiating endothelial cells. He also reflects on mentorship in Boston's vibrant research community and the excitement surrounding advancements in cell therapies.
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INSIGHT

Vasculature As A Master Regulator

  • Blood vessels are central signaling hubs that guide organ development, stem cell niches, and regeneration beyond simple nutrient delivery.
  • Juan Melero-Martin argues that making competent endothelial and mural cells is foundational for many regenerative therapies.
INSIGHT

Mitochondrial Transfer Boosts Engraftment

  • Mesenchymal stromal cells support transplanted endothelial cells via mitochondrial transfer through tunneling nanotubes.
  • Blocking mitochondrial transfer impairs endothelial survival, showing transfer is a major mechanism of support.
ADVICE

Prime Endothelial Cells With Mitochondria

  • Prime endothelial cells with isolated mitochondria before transplant to increase ATP production and stress resistance.
  • Use mitochondrial uptake to improve survival during the critical early, non-perfused period after implantation.
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