Sanger Sequencing in Clinical Infectious Diseases

It's a relatively new area so we don't have all the answers to the questions you might have. We did recently publish a couple of articles that might be of interest. The most recent one is in clinical infectious diseases this year and we did a look back on our 16s ribosomal RNA gene PCR sequencing assay applied to 2146 normally sterile tissue and body fluid samples. If the CT value is high we know that sequencing by and large doesn't give us a useful result so we just report that result as negative.