Do Reactive Oxidation Stress Cause Sleep Loss?

If you a fly, go without sleep, or are forced to go without sleep for too long, you're going to build up very abnormally high levels of these reactive oxygen species in your gut. And we saw it in flies, and we saw it with every method of sleep loss that we could think of. So then what we wanted to do was to basically, through genetic manipulations, only do this in the gut. But did i happen, any kind of faith that that would work? I mean, it really seemed like a phantasy. You know? It was absolutely shocking. As long as we get rid of these things in the gut, animals can survive.