Vitamin B3 is important to make nicotinamide adenine dinucleotide. It's called NAD plus, which has a positive charge associated with it. What this does is steal a hydrogen from glycerolidhyde 3 phosphate and becomes NADH plus. This makes the cytoplasm slightly acidic. So what is the equation here? NAD plus turns into NADH plus hydrogen ion,. A proton is simply H plus; one electron is neutral.