CRISPR Mitochondrial Knockout Library

In this week’s episode of The Energy Code, Dr. Mike explores one of the most eye-opening frontiers in modern biology: the mitochondrial roots of psychiatric disorders and cancer. Drawing from The Life Machines, he discusses research showing that mitochondrial dysfunction may drive anxiety and depression rather than simply correlate with them. Studies in animals demonstrate that altering mitochondrial fusion proteins like MFN2 can erase or induce anxiety-like behaviors, while human data links major depressive disorder to elevated mitochondrial DNA, impaired ATP production, and shortened telomeres. As psychiatrist Chris Palmer argues in the excerpt, mental illness may ultimately be a metabolic disorder of the brain—placing mitochondria at the center of emotional and cognitive health. 

 

Dr. Mike then shifts into a striking breakdown of how cancer hijacks mitochondrial biology to survive, evade immunity, and metastasize. From disabling programmed cell death to relying on hyper-fast glycolysis, to the astonishing ability to steal mitochondria from T-cells or poison them with damaged ones, cancer emerges as a metabolically cunning adversary. The episode concludes with a rapid review of the most compelling mitochondrial research from the past six months, including nanomaterials that supercharge stem-cell mitochondria, sprint training that breaks and rebuilds mitochondrial structure, the mitochondrial basis of T-cell memory and anti-tumor immunity, CRISPR tools revealing cancer’s metabolic vulnerabilities, mitochondrial drivers of sarcopenia, emerging blood biomarkers of mitochondrial aging, and groundbreaking three-parent IVF trials preventing inherited mitochondrial disease. It’s one of the most sweeping scientific episodes yet—revealing how mitochondrial medicine is rapidly reshaping our understanding of mental health, cancer, aging, and human vitality.

 

Key Points:

• Mitochondrial dysfunction may cause anxiety and depression, not just correlate with them.

• Modifying MFN2 levels in neurons can erase or induce anxiety-like behavior in animals.

• Depression in humans is linked to higher mtDNA, shorter telomeres, and reduced ATP efficiency.

• Cancer cells disable apoptosis, favor fast glycolysis, and hijack mitochondria from immune cells.

• Cancer can poison T-cells with damaged mitochondria and hide them from autophagy.

• Nano-materials can stimulate stem cells to overproduce mitochondria and transfer them to damaged cells.

• Sprint interval training breaks down mitochondria initially but drives stronger remodeling and efficiency.

• T-cell immunity and anti-tumor responses rely heavily on mitochondrial metabolic flexibility.

• CRISPR mitochondrial knockout screens reveal key metabolic weaknesses in cancer.

• Sarcopenia is rooted in impaired mitophagy and dysfunctional mitochondrial dynamics.

• A new blood mitochondrial biomarker correlates with fitness, aging, and cognitive risk.

• Three-parent IVF using donor mitochondria can prevent inherited mitochondrial disease.

 

Key Quotes from Dr. Mike

 

“Mental disorders… are metabolic disorders of the brain, driven by mitochondria.”

 

“Changes in the mitochondria impair communications between neurons, meaning treating the mitochondria may decrease the burden of psychiatric disorders.”

 

“Those sneaky cancer cells literally suck the life out of the cells that come to fight them.”

 

“Cancer cells not only steal mitochondria, they weaponize damaged ones to cripple the immune system.”

 

“The future of mitochondrial medicine isn’t the future — it’s now.”

 

Key Moments

 

00:00 – 00:03:36

Intro to the episode and setup for the discussion. 

 

00:03:36 – 00:11:37

Psychiatric Disorders & Mitochondria — Reading from The Life Machines covering MFN2, anxiety/depression mechanisms, mtDNA changes, telomere shortening, ATP reduction, and the metabolic roots of mental illness.

 

00:11:37 – 00:13:17

Product update inserted into the episode (BioLithin / transition section).

 

00:13:17 – 00:22:15

Cancer & Mitochondria — How cancer evades apoptosis, relies on glycolysis, steals mitochondria, poisons immune cells, and adapts metabolically during metastasis.

 

00:22:15 – 00:23:19

Transition into recent mitochondrial research roundup.

 

00:23:19 – 00:25:07

Study 1: Nano-Flowers & Mitochondrial Biogenesis — Nanomaterials doubling mitochondrial output and transferring mitochondria to damaged cells.

 

00:25:07 – 00:28:05

Study 2: Sprint Interval Training — Mitochondrial breakdown → remodeling → improved oxidative function.

 

00:28:05 – 00:32:09

Study 3: T-Cell Mitochondrial Metabolism — T-cell memory, exhaustion, and antitumor immunity tied to mitochondrial flexibility.

 

00:32:09 – 00:34:07

Study 4: Mitochondrial Knockout CRISPR Library — Identifying cancer vulnerabilities in mitochondrial antioxidant pathways.

 

00:34:07 – 00:38:04

Study 5: Sarcopenia & Aging Muscle — Impaired mitophagy and dysfunctional mitochondrial dynamics driving muscle loss.

 

00:38:04 – 00:41:10

Study 6: Blood-Based Mitochondrial Biomarkers — Links to VO₂ max, aging, dementia risk, and sex-specific mitochondrial differences.

 

00:41:10 – 00:45:04

Study 7: Three-Parent IVF & Mitochondrial Donation — Preventing inherited mitochondrial disease in newborns.

 

00:45:04 – 00:47:47

Discussion on the rapid evolution of mitochondrial medicine and future outlook.

    Books & References From Episode
    The Life Machines — by Daria Mochly-Rosen & Emmanuel Rosen

  Nanomaterials & Mitochondrial Biogenesis     Sprint Interval Training & Mitochondrial Remodeling     T-Cell Immunity & Mitochondrial Metabolism     CRISPR Mitochondrial Knockout Library     Sarcopenia & Mitochondrial Dysfunction     Blood-Based Mitochondrial Biomarker     Three-Parent IVF / Mitochondrial Donation     Stay Connected

 

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