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The Origins of Sexual Selection
Charles Darwin wrote that during the season of love, a musky odor is emitted by the glands of the crocodile and pervades their haunt. The problem was quantities were so small they couldn't be identified with chemistry of the time. A German chemist who had already been awarded the Nobel Prize for his work on the structure of hormones spent about 20 years trying to identify the sex pheromone of the silk moth. He then isolated the molecule from half a million silk moths,. identified it, synthesized it, and next crucial thing was using that bioassay, he went back to the male moths and said, have I got this female sex pherome right?